Custom Monoclonal Antibody

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Custom Monoclonal Antibody - $3,999 (complete service)

Phase Time (Cost)

Phase 1: Immunization

1. Immunization of Balb/c mice (up to 5 animals) with antigen to develop antiserums.

The client may choose to follow our standard immunization protocol or to use your own protocol. In most cases, we injected the mouse every two weeks for a total of 6 weeks before doing a test bleed on the mouse. The test bleed samples are then examined for antibody production using ELISA (The client may request up to 0.5 ml serum sample/per mouse for their own additional characterization). ELISA will be used to determine the highest titer mice to be used for cell fusion. The immunization process may be extended if the test result is not optimal.

Antigen requirement:

  0.5 mg of purified recombinant protein or intact protein will be required for immunization.

  With peptide synthesis request: Custom Peptide synthesis up to 15 amino acids (additional amino acid for $12 per residue) with HPLC purification up to 90% purity,  peptide KLH conjugation for immunization,  peptide BSA conjugation for screen. 10 mg free peptide will be available for the client.

8-10 weeks ($1,000 without peptide synthesis)


($1,500 with peptide synthesis)

Phase 2:

Fusion of spleen cells with myeloma cell line. The fused cells are then placed in 96-well culture plates. Viable colonies appear as early as 3-5 days after fusion and are kept healthy with a fresh supply of nutrient media as necessary

2-6 weeks $1,000

Phase 3:

When hybridoma colonies are large enough to screen, screening of all wells containing fusion products is done with ELISA assay. All positive wells will be expanded and frozen for parental hybridomas. The number of positives depends on the nature of the antigen, and can range from a few hybridomas to a dozen or more. Additional hybrids can be frozen for further studies.

2-6 weeks $1,000

Phase 4: Stabilization and Expension of clones

After positive hybridomas are selected for further development, they will be cloned using the limited dilution method and screened by ELISA. 5 clones with the desired specificity will be expanded, and hybridoma medium (50 - 100 ml) will be collected for further studies

2-4 weeks $1,000


17-29 weeks ($3,999)
Additional Options


The secreted antibody will be purified by Protein G affinity chromatography and deliver to the customer (Approximately 20-30 mg).
Antibodies are eluted using both pH11 and pH2.5 buffers and neutralized immediately followed by dialysis in PBS

1 week
$500/per project
Antagene Inc will provide the customer with the service in the shortest possible time.  Delays may be incurred if the antigen is poorly immunogenic .

All the products of this project shall become the exclusive property of the client at the conclusion of the project. Full confidentiality is guaranteed.

Please contact us for a quotation of your customized monoclonal antibody development project or

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